Unique Molecular Indexes (UMIs)
Most QIAseq library kits use Unique Molecular Indexes (UMIs) to improve performance.
UMIs can be used to identify and correct sequencing errors to allow higher sensitivity in variant calling.
UMIs can also be used to eliminate library amplification and sequencing biases in RNA quantification.
The Biomedical Genomics Analysis plugin has a number of tools for handling sequencing data from UMI based protocols:
- Remove and Annotate with Unique Molecular Index (
)
- Calculate Unique Molecular Index Groups (
)
- Create UMI Reads from Grouped Reads (
)
- Create UMI Reads from Reads (
)
- Create UMI Reads for miRNA (
)
Subsections