UMI group sizes

The tools Calculate Unique Molecular Index Groups, Create UMI Reads from Reads and Create UMI Reads for miRNA all finds UMI groups, i.e. reads originating from the same fragment. An important set of QC metrics which is common for all three tools regards the sizes of the UMI groups.

The reports from the tools all contain a Group table with the following information:

In addition, the following plots are also available:

For most applications the ideal UMI group size will be around 2-4, larger UMI groups tend to have diminishing returns for the increased sequencing budget. Please refer to the kit handbook to see the suggested UMI group size for your application.