- Primer sequence list. A sequence list with the created primers.
- Missing primers table. A table that lists information about rejected primers that did not fulfil the criteria including an explanation about why the primer was not created.
- Primer table. A table with information about each primer. If several primers are valid according to the requirements, the primer with the best score is used (see section 6.1.2).
- The input objects. The input supplied to the tool, with primers annotated on the sequence.
The primers created are the best possible according to the given parameters. If no primers are created another attempt can be made after making adjustments to some of the primer settings or the input sequence. Figure 6.10 shows an example of primers designed to a region containing a scaffold.