Nested PCR output table

In nested PCR there are four primers in a solution, forward outer primer (FO), forward inner primer (FI), reverse inner primer (RI) and a reverse outer primer (RO).

The output table can show primer-pair combination parameters for all four combinations of primers and single primer parameters for all four primers in a solution (see section on Standard PCR for an explanation of the available primer-pair and single primer information).

The fragment length in this mode refers to the length of the PCR fragment generated by the inner primer pair, and this is also the PCR fragment which can be exported.