Options for clc_quality_trim
usage: clc_quality_trim [options]
Trim a read file based on quality.
Options:
-h / --help: Display this message.
-r <file> / --input <file>: Input read file. (Required)
-q <file> / --quality <file>: Specify separate input quality file.
-o <file> / --output <file>: Output read file (fasta or fastq format depending
on name). (Required)
-p <file>/ --paired <file>: Output file for pairs (fasta or fastq).
-c <n> / --cutoff <n>: Set the minimum quality for a good nucleotide. (Default
20)
-b <n> / --badfraction <n>: Set the maximum fraction of bad nucleotides to
define a good quality region. (Default 0.1)
-l <n> / --lengthfraction <n>: Set the fraction of the read that must be of
good quality. (Default 0.5)
-m <n> / --minlength <n>: Set the minimum length of output reads. (Default 0)
-n / --notrim: Do not trim the sequence, but replace bad quality with Ns
instead.
-s / --colorspace: The data are from color space sequencing. This option
moves the start trim one position to the left (see manual).
-f <offset> / --qualityoffset <offset>: Set the ascii offset value in fastq
files (default is 64).
-i <file1> <file2> / --interleave <file1> <file2>: Interleave two sequence
files with the same number of sequences. May be used instead of a single
file.
