Options for clc_quality_trim
usage: clc_quality_trim [options] Trim a read file based on quality. Options: -h / --help: Display this message. -r <file> / --input <file>: Input read file. (Required) -q <file> / --quality <file>: Specify separate input quality file. -o <file> / --output <file>: Output read file (fasta or fastq format depending on name). (Required) -p <file>/ --paired <file>: Output file for pairs (fasta or fastq). -c <n> / --cutoff <n>: Set the minimum quality for a good nucleotide. (Default 20) -b <n> / --badfraction <n>: Set the maximum fraction of bad nucleotides to define a good quality region. (Default 0.1) -l <n> / --lengthfraction <n>: Set the fraction of the read that must be of good quality. (Default 0.5) -m <n> / --minlength <n>: Set the minimum length of output reads. (Default 0) -n / --notrim: Do not trim the sequence, but replace bad quality with Ns instead. -s / --colorspace: The data are from color space sequencing. This option moves the start trim one position to the left (see manual). -f <offset> / --qualityoffset <offset>: Set the ascii offset value in fastq files (default is 64). -i <file1> <file2> / --interleave <file1> <file2>: Interleave two sequence files with the same number of sequences. May be used instead of a single file.