Options for clc_mapper_legacy
usage: clc_mapper_legacy <options> Map some reads to some reference sequences. Options: -h / --help: Display this message -q / --reads: The files following this option are read files. (may be used several times) -d / --reference: The files following this option are reference files. Fasta and GenBank formats are allowed. (may be used several times) -o <file> / --output <file>: Output mapping result to a cas file (required) -i <file1> <file2> / --interleave <file1> <file2>: Interleave the sequences in two files immediately following the "-i" option, alternating between the two files when reading the sequences. Only valid for read files. (may be used several times). -x <n> / --mismatchcost <n>: Set the mismatch cost (range 1 to 3, default 2) -g <n> / --gapcost <n>: Set the gap cost (range 1 to 3, default 3) -e <n> / --deletioncost <n>: Set the deletion cost in which case the gap cost setting only applies to insertions. (range 1 to 3, default 3) -c / --colorspace: Use color space when aligning. -y <n> / --colorerrorcost <n>: Set the cost of an error in a color when using color space. Can only be used with the "-c" option. (range 1 to 3, default 3) -r <mode> / --repeat <mode>: Set the behavior for reads that match more than once, i.e. ignore such reads or place them randomly among the valid locations (ignore / random) (default random) -l <n> / --lengthfraction <n>: Set the fraction of the read that must match. A real number between 0.0 and 1.0 (default 0.5). -s <n> / --similarity <n>: Set the limit for the similarity in the fraction of the read that must match (according to "-l" option). A real number between 0.0 and 1.0 (default 0.8). -p <par> / --paired <par>: Set the paired read mode for the read files following this option. (may be used several times) par consists of four strings: <mode> <dist_mode> <min_dist> <max_dist> mode is ff, fb, bf, bb and sets the relative orientation of read one and two in a pair (f = forward, b = backward) dist_mode is ss, se, es, ee and sets the place on read one and two to measure the distance (s = start, e = end) A typical use would be "-p fb ss 180 250" which means that the reads are inverted and pointing towards each other. The distance includes both the reads and the sequence between them. The distance may be between 180 and 250, both included. To explicitly say that the following reads are not paired, use "no" for par, i.e. "-p no". For paired end reads split in two files, use the -i option. -m <n> / --memory <n>: Set the maximum amount of memory to use as a fraction of the available memory (default is 1.0). -t <n> / --maxalign <n>: Set the maximum number of alignments to report for each read (default is 1). -a <mode> / --alignmode <mode>: Set the alignment mode to one of the following: local: perform local alignment (default) global: perform global alignment semi-global: perform semi-global alignment -f / --forwardonly: Only match reads in the forward direction (cannot be used with paired data). --cpus <n>: Set the number of cpus to use. --no-progress: Disable progress bar. Examples: Map reads in a single file to a single file with reference sequences: clc_mapper_legacy -o mapping.cas -q reads.fasta -d reference.fasta Map reads from two unpaired runs and a paired end run split across two files. Use two reference sequences: clc_mapper_legacy -o mapping.cas -q unpaired1.fasta unpaired2.fasta -p fb ss 180 250 -i paired_1.qf paired_2.qf -d reference1.gb reference2.gb