Build Functional Profile

To compute the number of reads in a sample mapping to regions involved with Pfam domains or GO terms, you can run the Build Functional Profile tool by going to:

        Functional Analysis (Image functional_analysis_folder_closed_16_n_p) | Build Functional Profile (Image build_functional_profile_16_n_p)

In the first wizard (figure 15.11), select the read mapping for which you want to build the functional profile.

Image createfunpro
Figure 15.11: Select a read mapping.

The parameters that can be set are seen in figure 15.12:

Image createfunpro1
Figure 15.12: Specify a reference and a GO database.

You can then select which output elements should be generated figure 15.13.

Image createfunpro2
Figure 15.13: Specify what type of output you want the tool to generate.

The resulting functional abundance tables store the number of reads corresponding to each Pfam domain, GO term, best BLAST hit or best DIAMOND hit.