Prepare sequencing data
The first thing to do after data import is to check the quality of the sequencing reads and perform the necessary trimming. This applies no matter whether you are working with Whole Genome Sequencing, Whole Exome Sequencing, Targeted Amplicon Sequencing or Whole Transcriptome Sequencing. In the toolbox you can choose between the two different ready-to-use workflows for data preparation that are shown in the "Run workflow 1" box in figure 12.19.
Subsections
- Choosing between Prepare Raw Data and Prepare Overlapping Raw Data workflows
- Import adapter trim list
- How to run the Prepare Overlapping Raw Data ready-to-use workflow
- How to run the Prepare Raw Data ready-to-use workflow
- Output from the Prepare Overlapping Raw Data and Prepare Raw Data workflows
- How to check the output reports