MLST quick-start
This section briefly describes how to get started using the main functionality of the QIAGEN CLC MLST Module.First, download an MLST Scheme:
Multilocus Sequence Typing (
) |
MLST Schemes (
) | Download MLST
Schemes (PubMLST) (
)
In this dialog, select the correct scheme to use as reference for
typing. Save the scheme (
).
Next, to find a sequence type for your sequencing data, you will have to create an isolate:
Multilocus Sequence Typing () |
Assembly and Create Isolate (
)
In this dialog, select all the sequencing data for the isolate that
you wish to type. (Note that you will have to
Import (
) the data first.)
Click Next and select the scheme (
) that you
downloaded as reference scheme. Click Next through a series of
steps where sequences are assigned to each locus in the scheme. If
the sequence is not automatically assigned correctly, use the
Add (
) and Remove (
) buttons to make sure
the right sequences are assigned.
When you click Finish, an isolate is created and a sequence type is assigned. Double-click a row in the upper part of the view to inspect and edit the contig.