CLC Main Workbench
Restriction enzyme lists
includes all the
restriction enzymes available in the REBASE
database, with methylation shown as performed by the cognate methylase rather than by Dam/Dcm. If you want to customize the enzyme database for your installation, see Restriction enzymes database configuration
However, when performing restriction site analyses, it is often an
advantage to use a customized list of enzymes. In this case, the
user can create special lists containing for example all enzymes available
in the laboratory freezer, or all enzymes used to create a given
restriction map or all enzymes that are available form the preferred
In the Example data
under Nucleotide->Restriction analysis, there are two enzyme lists:
one with the 50 most popular enzymes, and another with all enzymes
that are included in the CLC Main Workbench.
This section describes how you can create an enzyme list, and how
you can modify it.