Create enzyme list
CLC Main Workbench uses enzymes from the REBASE restriction enzyme database at http://rebase.neb.com. If you want to customize the enzyme database for your installation, see Restriction enzymes database configuration.
To create an enzyme list of a subset of these enzymes:
File | New |
Enzyme list (
)
This opens the dialog shown in figure 20.49
Figure 20.49: Choosing enzymes for the new enzyme list.
At the top, you can choose to Use existing enzyme list. Clicking this option lets you select an enzyme list which is stored in the Navigation Area. A list of popular enzymes is available in the Example Data folder you can download from the Help menu.
Below there are two panels:
- To the left, you can see all the enzymes that are in the list selected above. If you have not chosen to use an existing enzyme list, this panel shows all the enzymes available.
- To the right, you can see the list of the enzymes that will be used.
Select enzymes in the left side panel and add them to the right
panel by double-clicking or clicking the Add button
(
). If you e.g. wish to use EcoRV and BamHI, select these
two enzymes and add them to the right side panel.
If you wish to use all the enzymes in the list:
Click in the panel to the left | press Ctrl + A
(
+ A on Mac) | Add ()
The enzymes can be sorted by clicking the column headings, i.e. Name, Overhang, Methylation or Popularity. This is particularly useful if you wish to use enzymes which produce a 3' overhang. In this case, you can sort the list by clicking the Overhang column heading, and all the enzymes producing 3' overhangs will be listed together for easy selection.
When looking for a specific enzyme, it is easier to use the Filter. If you wish to find e.g. HindIII sites, simply type HindIII into the filter, and the list of enzymes will shrink automatically to only include the HindIII enzyme. This can also be used to only show enzymes producing a blunt cut site as shown in figure 20.50.
Figure 20.50: Selecting enzymes.
If you need more detailed information and filtering of the enzymes, either place your mouse cursor on an enzyme for one second to display additional information (see figure 20.51), or use the view of enzyme lists (see Restriction enzyme list).
Figure 20.51: Showing additional information about an enzyme like recognition sequence or a list of commercial vendors.
Click Finish to open the enzyme list.