• Product manuals

Browse the manual

• Introduction
• General Description
  • Supported sample kits
  • Accessing RNA-seq Analysis Portal
  • Analysis credits for RNA-seq Analysis Portal
• Getting Started
  • Upload sequencing data
  • Align and count
  • Create experiment
  • View and filter results
  • What's next - sending results to GeneGlobe
  • Next steps in GeneGlobe
• User Interface
  • Analysis Portal page
    • Front page and Projects overview
    • Project view
    • Send copy of project
  • Samples page
• Starting Analyses
  • Align and count dialog
    • QIAseq UPX 3' Transcriptiome Kit and QIAseq UPXome RNA Lib Kit specific settings
  • Create experiment dialog
    • Sample grouping
    • Experimental design
  • Compare analyses dialog
• Viewing Results
  • Experiment summary and QC report
  • Differential expression view
    • Feature table
    • Volcano plot
    • Heat map
    • Biological insights table
    • Filter
    • What's next
  • Comparison view
• RNA-seq Analysis Portal analysis explained
  • Align and count analysis workflows
    • Align and count - QIAseq miRNA Library Kit
    • Align and count - QIAseq UPX 3' Transcriptome Kit
    • Align and count - QIAseq UPXome RNA Lib Kit
    • Align and count - Demultiplexed RNA sample kits
  • Create experiment analysis workflow
  • Taxonomic profiling of unmapped reads
  • Compare analyses
• Appendix A - Sequence file naming pattern
• Appendix B - Reference and annotation information
  • General references and annotations
  • Sample kit specific resources
• Appendix C - Analysis workflow versions, species support and parameters
  • QIAseq miRNA Library Kit
  • QIAseq UPX 3' Transcriptome Kit
  • QIAseq UPXome RNA Lib Kit
  • QIAseq UPXome RNA Lib Kit (N6-T RT primer)
  • QIAseq UPXome RNA Lib Kit (ODT-T RT primer)
  • QIAseq UPXome RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq FastSelect RNA Lib Kit (N6-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (ODT-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq Stranded mRNA Select/Stranded Total RNA Lib Kit
  • TruSeq Stranded Total RNA Library Prep (Human/Rat, Gold, Globin)
  • Illumina Stranded Total RNA Prep with Ribo-Zero Plus
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
  • KAPA RNA HyperPrep Kit
  • SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian/Low Input Mammalian
  • Collibri Stranded RNA Library Prep Kit for Illumina Systems

What's next - sending results to GeneGlobe

When you have saved a filter, you can send the results to GeneGlobe Whats Next - Follow-up Experiment Planner that will assist you in identifying products for follow-up experiments.

1. Select the filter of choice (figure 14).
2. In the What's next box, click on the Start now button. This sends the filtered list of features and the full lists of Canonical Pathways, and - when applicable - Upstream regulator (miRNA) to GeneGlobe Whats Next - Follow-up Experiment Planner.

   GeneGlobe Whats Next - Follow-up Experiment Planner opens up in a new tab. Depending on your browser settings, you may need to first allow pop-ups.

Figure 14: The Filter and What's next sections from where you can save filters and send results to GeneGlobe.

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