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• Introduction
• General Description
  • Supported sample kits
  • Accessing RNA-seq Analysis Portal
  • Analysis credits for RNA-seq Analysis Portal
• Getting Started
  • Upload sequencing data
  • Align and count
  • Create experiment
  • View and filter results
  • What's next - sending results to GeneGlobe
  • Next steps in GeneGlobe
• User Interface
  • Analysis Portal page
    • Front page and Projects overview
    • Project view
    • Send copy of project
  • Samples page
• Starting Analyses
  • Align and count dialog
    • QIAseq UPX 3' Transcriptiome Kit and QIAseq UPXome RNA Lib Kit specific settings
  • Create experiment dialog
    • Sample grouping
    • Experimental design
  • Compare analyses dialog
• Viewing Results
  • Experiment summary and QC report
  • Differential expression view
    • Feature table
    • Volcano plot
    • Heat map
    • Biological insights table
    • Filter
    • What's next
  • Comparison view
• RNA-seq Analysis Portal analysis explained
  • Align and count analysis workflows
    • Align and count - QIAseq miRNA Library Kit
    • Align and count - QIAseq UPX 3' Transcriptome Kit
    • Align and count - QIAseq UPXome RNA Lib Kit
    • Align and count - Demultiplexed RNA sample kits
  • Create experiment analysis workflow
  • Taxonomic profiling of unmapped reads
  • Compare analyses
• Appendix A - Sequence file naming pattern
• Appendix B - Reference and annotation information
  • General references and annotations
  • Sample kit specific resources
• Appendix C - Analysis workflow versions, species support and parameters
  • QIAseq miRNA Library Kit
  • QIAseq UPX 3' Transcriptome Kit
  • QIAseq UPXome RNA Lib Kit
  • QIAseq UPXome RNA Lib Kit (N6-T RT primer)
  • QIAseq UPXome RNA Lib Kit (ODT-T RT primer)
  • QIAseq UPXome RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq FastSelect RNA Lib Kit (N6-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (ODT-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq Stranded mRNA Select/Stranded Total RNA Lib Kit
  • TruSeq Stranded Total RNA Library Prep (Human/Rat, Gold, Globin)
  • Illumina Stranded Total RNA Prep with Ribo-Zero Plus
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
  • KAPA RNA HyperPrep Kit
  • SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian/Low Input Mammalian
  • Collibri Stranded RNA Library Prep Kit for Illumina Systems

Getting Started

RNA-seq Analysis Portalguides you through your RNA expression analysis in 5 steps:

1. Upload sequencing data
2. Align and count
3. Create experiment
4. Filter
5. What's next

When you log into RNA-seq Analysis Portal, you will find steps 1-3 at the top of the Analysis Portal page (figure 3).

Your analysis credits, including the number reserved for running analyses, are displayed in the top right corner, just under your name.

Figure 3: Analysis steps 1 to 3 are accessed from the top of the Analysis Portal page. This is also where you find your analysis credit balance.

For first-time users, onboarding pop-ups will appear to describe functionality and guide new users through the flow of uploading, aligning, creating experiments and sending the results back to GeneGlobe. You can click the "Don't show introductions tips" to stop the dispaly of these helper tips, but just close it to see more tips on later stages. If you want to re-enable these pop-ups this option is available from the 'Help' menu.

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Subsections

• Upload sequencing data
• Align and count
• Create experiment
• View and filter results
• What's next - sending results to GeneGlobe
• Next steps in GeneGlobe

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