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• Introduction
• General Description
  • Supported sample kits
  • Accessing RNA-seq Analysis Portal
  • Analysis credits for RNA-seq Analysis Portal
• Getting Started
  • Upload sequencing data
  • Align and count
  • Create experiment
  • View and filter results
  • What's next - sending results to GeneGlobe
  • Next steps in GeneGlobe
• User Interface
  • Analysis Portal page
    • Front page and Projects overview
    • Project view
    • Send copy of project
  • Samples page
• Starting Analyses
  • Align and count dialog
    • QIAseq UPX 3' Transcriptiome Kit and QIAseq UPXome RNA Lib Kit specific settings
  • Create experiment dialog
    • Sample grouping
    • Experimental design
  • Compare analyses dialog
• Viewing Results
  • Experiment summary and QC report
  • Differential expression view
    • Feature table
    • Volcano plot
    • Heat map
    • Biological insights table
    • Filter
    • What's next
  • Comparison view
• RNA-seq Analysis Portal analysis explained
  • Align and count analysis workflows
    • Align and count - QIAseq miRNA Library Kit
    • Align and count - QIAseq UPX 3' Transcriptome Kit
    • Align and count - QIAseq UPXome RNA Lib Kit
    • Align and count - Demultiplexed RNA sample kits
  • Create experiment analysis workflow
  • Taxonomic profiling of unmapped reads
  • Compare analyses
• Appendix A - Sequence file naming pattern
• Appendix B - Reference and annotation information
  • General references and annotations
  • Sample kit specific resources
• Appendix C - Analysis workflow versions, species support and parameters
  • QIAseq miRNA Library Kit
  • QIAseq UPX 3' Transcriptome Kit
  • QIAseq UPXome RNA Lib Kit
  • QIAseq UPXome RNA Lib Kit (N6-T RT primer)
  • QIAseq UPXome RNA Lib Kit (ODT-T RT primer)
  • QIAseq UPXome RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq FastSelect RNA Lib Kit (N6-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (ODT-T RT primer)
  • QIAseq FastSelect RNA Lib Kit (N6-T + ODT-T RT primers)
  • QIAseq Stranded mRNA Select/Stranded Total RNA Lib Kit
  • TruSeq Stranded Total RNA Library Prep (Human/Rat, Gold, Globin)
  • Illumina Stranded Total RNA Prep with Ribo-Zero Plus
  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
  • KAPA RNA HyperPrep Kit
  • SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian/Low Input Mammalian
  • Collibri Stranded RNA Library Prep Kit for Illumina Systems

Analysis credits for RNA-seq Analysis Portal

To run the initial Align and count analysis in RNA-seq Analysis Portal you will need analysis credits.

You can add analysis credits to your account using either the lot number of your QIAseq RNA sample kit or an analysis credit code following the steps below. If you will be using a QIAseq RNA sample kit lot number, you can skip the step Purchase analysis credits.

1. Purchase analysis credits. You can purchase analysis credits on https://geneglobe.qiagen.com/analyze/analysis-credits. Choose between GeneGlobe Analyze RNA-seq (12), or GeneGlobe Analyze RNA-seq (24). Once you have placed your order, you will receive an email with the analysis credit code.
2. Redeem analysis credits. You can redeem analysis credits using a lot number from one of the supported QIAseq RNA sample kits or an analysis credit code at https://geneglobe.qiagen.com/analyze/analysis-credits/redeem-credits. For instructions on how to locate the lot number, see figure 2.

Figure 2: The lot number is on the label of your RNA sample kit box.

Your analysis credit balance and credit history is provided on the QIAGEN GeneGlobe Data Analysis Center dashboard.

Your analysis credit balance is also shown at the top, right side of the Analysis Portal page, described in the chapter Getting Started.

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