Correct Long Reads output
In addition to the sequence list of corrected reads, a summary report is available via the output option Create report.
Correct Long Reads report
An example of the top part of the Correct Long Reads summary report is shown in figure 7.2.
Figure 7.1: Correct Long Reads report.
The report is divided into two sections, Input statitics and Output statitics, both containing the following information:
- Reads. Number of reads.
- Minimum, Maximum, Average. Minimum, maximum and average sequence length.
- N50. The length of the shortest sequence in sets of sequences of equal length or longer, where the summed length of sequences is at least 50% of the total sequence length.
- N90. The length of the shortest sequence in a set of sequences of equal length or longer, where the summed length of sequence is at least 90% of the total sequence length. N90 will be equal to or smaller than N50.
- Total. The number of bases in the sequences.
- Sequence length distribution. A graph depicting the number of sequences found at a specific length.