Supplementary QC Report
- 1 Summary
- 2 Per-sequence analysis: Lengths distribution; GC-content; Ambiguous base-content; Quality distribution
- 3 Per-base analysis: Coverage; Nucleotide distributions; GC-content; Ambiguous base-content; Quality distribution
- 4 Over-representation analyses: Enriched 5mers; Sequence duplication levels; Duplicated sequences
For a detailed description of the QC reports and indication on how to interpret the different values, see http://clcsupport.com/biomedicalgenomicsworkbench/current/index.php?manual=Report_contents.html.
If you can accept the read quality you can now proceed to the next step and use the prepared reads output as input in the next ready-to-use workflow. If the quality of your reads is poor and cannot be accepted for further analysis, the best solution to the problem is to go back to start and resequence the sample.
You are now ready to perform the actual analysis of your sequencing data (see figure 12.37).
Figure 12.37: Use the prepared data as input in the relevant ready-to-use workflow, which we here for the sake of simplicity call "Workflow 2".