Interpreting the results
The Create Fold Change Track tool will produce an annotation track, including the genes or transcripts that were not filtered out based on the filtering criteria. Each feature in this track is annotated with the following information:
- Fold-change Represents the fold-change according to the specified scale. A positive value indicates that the expression level was higher in the case. A negative value indicates that the expression level was higher in the control. If Raw was specified for the scale parameter, a value of 0 represents no difference. If a logarithm-based value was specified for the scale parameter, a value of 1 represents no difference.
- Difference Represents the difference in expressions. A positive value indicates that the expression level was higher in the case. A negative value indicates the expression level was higher in the control.
- Maximum expression Represents the larger of the expression values observed in the case and the control samples.
- Expression (case) Gives the expression value in the case sample
- Expression (control) Gives the expression value in the control sample
As is the case with all annotation tracks in the Biomedical Genomics Workbench, it is possible to sort and filter the results based on any of the above values, and to create track lists for further analysis of the results.