Create Venn Diagram for RNA-Seq

The Create Venn Diagram tool makes it possible to compare the overlap of differentially expressed genes or transcripts in two or more statistical comparison tracks. The genes considered to be differentially expressed can be controlled by setting appropriate p-value and fold change thresholds.

To create the Venn diagram:

        Toolbox | RNA-Seq and Small RNA Analysis (Image expressionfolder)| Create Venn Diagram (Image venn_16_n_p)

Select a number of statistical comparison tracks (Image stats_track_16_n_p) and click Next (see figure 30.38).

Image venn_output
Figure 30.38: The resulting Venn diagram.

In the Side Panel to the right, it is possible to adjust the Venn Diagram settings. Under Layout, you can adjust the general properties of the plot.

The Data side panel group makes it possible to choose the differentially expressed genes or features of interest. The set of statistical comparisons to be compared can be selected using the drop down combo boxes at the top of the group. It is possible to customize the color of a given statistical comparison using the color picker next to the drop down combo box.

Finally, the Text format group makes it possible to adjust the settings for the count and statistical comparison labels.