View and edit contigs

The result of the assembly process is one or more contigs where the sequence reads have been aligned (see figure 18.20).

Image initialcontigview
Figure 18.20: The view of a contig. Notice that you can zoom to a very detailed level in contigs.

You can see that color of the residues and trace at the end of one of the reads has been faded. This indicates, that this region has not contributed to the contig. This may be due to trimming before or during the assembly or due to misalignment to the other reads.

You can easily adjust the trimmed area to include more of the read in the contig: simply drag the edge of the faded area as shown in figure 18.21.

Image dragtrimmedregion
Figure 18.21: Dragging the edge of the faded area.

Note! This is only possible when you can see the residues on the reads. This means that you need to have zoomed in to 100% or more and chosen Compactness levels "Not compact", "Low" or "Packed". Otherwise the handles for dragging are not available (this is done in order to make the visual overview more simple).

If reads have been reversed, this is indicated by red. Otherwise, the residues are colored green. The colors can be changed in the Side Panel as described in View settings in the Side Panel

If you find out that the reversed reads should have been the forward reads and vice versa, you can reverse complement the whole contig(imagine flipping the whole contig):

        right-click in the empty white area of the contig | Reverse Complement


Subsections