Metadata for RNA-Seq

The statistical analysis and visualization tools of the RNA-Seq folder make extensive use of the metadata system. For example, metadata are required when defining the experimental design in the Differential Expression for RNA-Seq tool, and can be used to add extra layers of insight in the Create Heat Map and PCA for RNA-Seq tools.

To get the most out of these tools we recommend that all input expression tracks have associated metadata, as shown in figure 28.24. For information about how to use and setup metadata, please see Metadata.

Image metadata_tissue
Figure 28.24: An example of expression tracks with associated metadata.