Supplementary QC Report
- 1 Summary
- 2 Per-sequence analysis
- 2.1 Lengths distribution
- 2.2 GC-content
- 2.3 Ambiguous base-content
- 2.4 Quality distribution
- 3 Per-base analysis
- 3.1 Coverage
- 3.2 Nucleotide distributions
- 3.3 GC-content
- 3.4 Ambiguous base-content
- 3.5 Quality distribution
- 4 Over-representation analyses
- 4.1 Enriched 5mers
- 4.2 Sequence duplication levels
- 4.3 Duplicated sequences
The majority of the reads should have a PHRED score above 30 when looking at the "Quality distribution" graph.
If you can accept the read quality you can now proceed to the next step and use the prepared reads output as input in the next ready-to-use workflow. If the quality of your reads is poor and cannot be accepted for further analysis, the best solution to the problem is to go back to start and resequence the sample.