Output from the Perform QIAseq Immune Repertoire Analysis workflow
Different outputs are generated, the key outputs being the TCR analysis report and the Clonotypes table. A folder called QC & Reports holds information about UMI grouping and trimming.
- TCR analysis report A report that summarizes statistics of the detected T cell repertoire.
See section 8.2.1 for at detailed description.
- Clonotypes A table presenting the clonotypes identified in the sample.
See section 8.2.1 for at detailed description.
- QC & Reports Folder that holds four different reports:
- Remove and annotate with UMI report A summary of total number of reads, total number of reads that have UMIs and the nucleotide counts for each unique molecular barcode position (for more information, please see Remove and Annotate with Unique Molecular Index).
- UMI read report A comprehensive summary report for the detected UMI reads with information about grouping, QC and read length distribution (For more information, please see Create UMI Reads from Reads).
- Trim reads report This report contains information about the trimming such as length of the reads before and after trimming, trim settings, a trim summary and detailed information about the trim results (for more information, please see http://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=Trim_output.html).
- Combined report This output is a report that combines the highlights from the three original reports described above as well as from the Immune Repertoire Analysis report (for more information, please see http://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/current/index.php?manual=Combine_Reports.html).
- Remove and annotate with UMI report A summary of total number of reads, total number of reads that have UMIs and the nucleotide counts for each unique molecular barcode position (for more information, please see Remove and Annotate with Unique Molecular Index).