# # REBASE version 012 embossa_e.012 # # =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= # REBASE, The Restriction Enzyme Database http://rebase.neb.com # Copyright (c) Dr. Richard J. Roberts, 2010. All rights reserved. # =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= # # Rich Roberts Nov 29 2010 # # REBASE enzyme patterns for EMBOSS (embossre.enz) # # Format: # # namepatternlenncutsbluntc1c2c3c4 # # Please note that 4 cutters are not supported in CLC Workbenches. # Entries to be made visible in the CLC Workbenches must have a 2 in the fourth column (ncuts) # and the final two columns must both be 0. # # Where: # name = name of enzyme # pattern = recognition site # len = length of pattern # ncuts = number of cuts made by enzyme # Zero represents unknown # blunt = true if blunt end cut, false if sticky # c1 = First 5' cut # c2 = First 3' cut # c3 = Second 5' cut - not supported in CLC Workbenches # c4 = Second 3' cut - not supported in CLC Workbenches # # Examples: # AAC^TGG -> 6 2 1 3 3 0 0 # A^ACTGG -> 6 2 0 1 5 0 0 # AACTGG -> 6 0 0 0 0 0 0 # AACTGG(-5/-1) -> 6 2 0 1 5 0 0 # (8/13)GACNNNNNNTCA(12/7) -> 12 4 0 -9 -14 24 19 - this form, with 4 cut sites, is not supported in CLC Workbenches # # i.e. cuts are always to the right of the given # residue and sequences are always with reference to # the 5' strand. # Sequences are numbered ... -3 -2 -1 1 2 3 ... with # the first residue of the pattern at base number 1. # # MyEnzI TTTTTT 6 2 0 5 1 0 0 MyEnzII TTTTTT 6 2 0 5 1 0 0