Number of cut sites
Clicking Next confirms the list of enzymes which will be
included in the analysis, and takes you to the dialog shown in
figure 19.42.
Figure 19.42: Selecting number of cut sites.
If you wish the output of the restriction map analysis only to include restriction enzymes which cut the sequence a specific number of times, use the checkboxes in this dialog:
- No restriction site ()
- One restriction site (1)
- Two restriction sites (2)
- Three restriction site (3)
- N restriction sites
- Minimum
- Maximum
- Any number of restriction sites > 0
You can use the checkboxes to perform very specific searches for restriction sites: e.g. if you wish to find enzymes which do not cut the sequence, or enzymes cutting exactly twice.