Output from primer design

The output generated by the primer design algorithm is a table of proposed primers or primer pairs with the accompanying information (see figure 19.6).

Image primertable_web
Figure 19.6: Proposed primers

In the preference panel of the table, it is possible to customize which columns are shown in the table. See Standard PCR, Nested PCR, TaqMan or Sequencing primers for a description of the available information.

The columns in the output table can be sorted by the present information. For example the user can choose to sort the available primers by their score (default) or by their self annealing score, simply by right-clicking the column header.

The output table interacts with the accompanying primer editor such that when a proposed combination of primers and probes is selected in the table the primers and probes in this solution are highlighted on the sequence.



Subsections