Metadata and RNA-Seq

The statistical analysis and visualization tools of the RNA-Seq folder make extensive use of the metadata system. For example, metadata are required when defining the experimental design in the Differential Expression for RNA-Seq tool, and can be used to add extra layers of insight in the Create Heat Map and PCA for RNA-Seq tools.

To get the most out of these tools we recommend that all input expression tracks are associated with metadata. Importing metadata and associated data elements with it is described in Metadata.

Image metadata_tissue
Figure 33.1: A metadata table with expression samples associated with it.