Create Primers output

The Create Primers tool creates four different outputs:

The primers created are the best possible according to the given parameters. If no primers are created another attempt can be made after making adjustments to some of the primer settings or the input sequence. Figure 5.10 shows an example of primers designed to a region containing a scaffold.

Image create_primers_to_annotated_scaffold2
Figure 5.10: Primers have been designed to a region containing a scaffold. Prior to primer creation, an amplicon has been created using the Create Amplicon Tool and annotated "For primer creation".